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ing contaminated. If you inject four sites, the culture has a .5 * .5 * .5 * .5 = 6% of NOT becoming contaminated. If you do everything right, this technique to increase your probability of producing a contamination (16 of 39) 5/1/2002 6:54:26 PM How To Grow Magic Mushrooms The Magic Mushroom Growers Guide (page 4) free culture should not be necessary. However, many people have problems generating sterile spore prints at the start of their cultivating experience and this will help those people continue to generate cultures until they get enough experience. The first time you use a spore syringe that you prepared yourself, you may want to inoculate half of your jars the normal way, and the other half this way. If your spore syringe is just a 'little' dirty, this will give you second chance to grow more mushrooms and prepare a cleaner spore syringe. Disadvantages: It will take significantly longer for the jar to become 100% colonized. Back to preparation and colonization of substrate. table of contents. Adaptation-5: Large amounts of Inoculate. You can speed up the colonization of a jar dramatically by simply injecting the substrate material with more inoculate. If you inject 1 cc of inoculate at each site, you will get many germination's and the cake will colonize significantly faster. You should place the beveled side of the syringe needle against the glass so that the inoculate is coming out of the syringe and heading towards the glass. It should form a thin puddle of liquid between the glass and the substrate. 1 cc of inoculate should produce a puddle several inches in diameter. Advantage: Normally, people want the substrate to colonize as quickly as possible. This will help accomplish that goal. Also, the sooner and more fully the cake gets colonized, the less chance there is that contamination will get a foot hold and destroy the cake. (17 of 39) 5/1/2002 6:54:26 PM How To Grow Magic Mushrooms The Magic Mushroom Growers Guide (page 4) Disadvantages: This adaptation requires extra inoculate. If you are producing your own spore syringes it is not a factor. A single spore print can produce many (close to 50) spore syringes. If you are purchasing your spore syringes, you may wish to wait the few extra days to avoid the extra cost of using more inoculate. Back to preparation and colonization of substrate. table of contents. Adaptation-6: 80 Degree Colonization Temperature The culture jars can be colonized at a temperature higher than room temperature. 80 Degrees F. is ideal. Slightly higher is OK. There are several easy ways to accomplish this. If you have a floor heater with a pilot light and it is summer time (so the heat is not going to come on), you might be able to put the cakes in a shoe box and set them on the unit. The top of your water heater might be a good candidate. You can fill a cake pan half way with water and put a submersible fish tank heater in the water set to 80 degrees. ayahuasca school Shiitake Brooklynella About Forum % PURE Lagochilin Plant trees recipe recipe Shiitake Brooklynella Ayahuasca Preparation