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Defatting is a very important step in the isolation of pure alkaloid.<br> The fats and oils present in the crop must be removed because if they were left in, a tenacious emulsion would form during the extraction of the alkaloid, and you could forget about ever getting even close to a pure amide extract. For all practical purposes, all that would be extracted would be garbage.<br> Defatting can be done with any one of several very common and easily available solvents. For a 200 pound crop, one can count on using at least one, and possibly two 55 gallon drums of solvent.<br> The defatting can be done with either hexane, petroleum ether (not ethyl ether) mineral spirits or naphtha.<br> The preferred procedure for small scale extractions is to put the ground-up, solvent-soaked crop into a burette, and then keep dripping fresh solvent onto the top of the material until the solvent coming out at the bottom of the burette does not leave a grease stain on filter paper when the solvent dries. 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