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be distracted by other matters while the product spoils.
There are several alternate procedures for the extraction of the
amides from ergot. They all produce roughly similar results. This is
fortunate, as it allows the acid chemist to choose the materials used
based upon availability rather than being rigidly locked into using a
certain set of materials.
The first step in the extraction procedure, regardless of whether
ergot or seeds are being extracted, is a thorough grinding.
A blender is
suitable for this job, and a coffee grinder may work as well if it gives a
fine grind. Once the crop has been ground up, it is immediately
vulnerable to attack by light and air, so as soon as it is ground it
should be wetted with the solvent chosen for use in the next step:
Defatting is a very important step in the isolation of pure alkaloid.
The fats and oils present in the crop must be removed because if they
were left in, a tenacious emulsion would form during the extraction of
the alkaloid, and you could forget about ever getting even close to a
pure amide extract. For all practical purposes, all that would be
extracted would be garbage.
Defatting can be done with any one of several very common and
easily available solvents. For a 200 pound crop, one can count on
using at least one, and possibly two 55 gallon drums of solvent. The
defatting can be done with either hexane, petroleum ether (not ethyl
ether) mineral spirits or naphtha. The preferred procedure for small
scale extractions is to put the ground-up, solvent-soaked crop into a
burette, and then keep dripping fresh solvent onto the top of the
material until the solvent coming out at the bottom of the burette does
not leave a grease stain on filter paper when the solvent dries.
easily scaled up for our 200 pound crop by replacing the burette with
clean pipes about 4 inches in diameter, and about 4 feet long, with
suitable valves and filters at the bottom to prevent everything from
falling out. (See Figure 1). When all the fats have been removed from
Extraction And Isolation Of
The Lysergic Acid Amides
the crop, the best procedure is to
evaporate the remaining defatting
solvent from the crop under a
This is not practical for
a large crop, so letting the
remainder drip out of the bed
over a period of a few hours is
With the fats removed, the
ergot alkaloids can be extracted
from the crop. Note here the
word alkaloid. This is the key to
all variations of the extraction
There is a piperidine
nitrogen atom in the lysergic
these molecules that possesses
basic properties similar to ammonia and amines. This atom allows
the lysergic molecules to form salts with acids, and also causes the
solubility characteristics of the molecule to change Mdmabath Salts For Sale
whether the molecule is in acid or basic solution. It further allows the
lysergic amides, including LSD, to form crystals from solution.
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cup of water for each 1/2 pint jar you plan to prepare.
If you are using 1 pint jars you need to double the recipe. Mix all of
this stuff up well. This mixture is the substrate material that the fungus
will consume and use for growth.
The next step is to fill each jar with substrate material. Adaptation-20
This document used to suggest gently packing the substrate into the
culture jars. It has been found that keeping the substrate as loose and
full of air as possible is the best way to fill the jars. The jars will
colonize faster this way. Incidentally, the faster the jar colonizes, the
lower the risk that some competitor contamination will get a foot hold
and take over the substrate. Adaptation-3 Fill each jar to within 1/2
inch of the top with substrate material. If you run out of substrate
material, either mix up enough for one more 1/2 pint jar or cannibalize
a jar to fill up the rest of the jars. This is important because you need to
make sure the substrate is high enough in the jars for the spore syringe
to inject spores into it.
The top 1/2 inch of the glass on each culture jar needs to be cleaned.
No substrate material can be left on the glass above the compressed
cake. First wipe it with your finger to get the bulk of the material off of
it and then do a thorough job with a moistened paper towel. The glass
needs to be spotless. The reason this is necessary is that bacteria and
mold can use any material left there as a wick to infect the main
(3 of 29) 5/1/2002 6:54:17 PM
How To Grow Magic Mushrooms The Magic Mushroom Growers Guide (page 2)
Next, fill the top 1/2 inch of the each culture jar with vermiculite. This
layer is pure, simple, dry vermiculite. Nothing else. Fill the jar level
with the glass edge. This layer is a break through pioneered by
Psylocybe Fanaticus. What this layer does is insulate the sterilized
substrate from any air borne contamination. This layer gets sterilized
with the substrate later and air borne molds and bacteria can not
(usually) get through it to contaminate the substrate. At the same time,
it allows some gas exchange to occur. The fungus needs oxygen and
gasses can filter through the vermiculite.
Now, place the jar lids in place. Normally, the jar lids have a rubber
seal that is placed in contact with the glass of the jar. Traditionally the
rubber seal is not placed in contact with the glass. It was placed on the
upper side of the lid. The reason was that people thought it would
make too tight of a seal. This does not seem to be an issue. If you wish
to follow tradition, place the rubber on the upper side of the lid. Screw
the lid down tight. Note that you need to have the four holes poked in
the lid in Step 1. Otherwise you can have real problems when you heat
these jars up!
Next, place a piece of tin foil over the top of each jar and crumple it
around the sides of the jar. This is to keep water dro
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