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Emiliano Eva, born in 1978, was a musician Tronchin Denis, born in 1977, was a graphic designer (web designer). Both were flown to Milan to Quito July 28, 2006 to determine the Shuar Indian shamanic practices and experience ayahuasca (or yagé) (1). They had to be back in Italy on August 19. Denis became interested in shamanism surfing the Internet. Through the website "The Drum of the shaman," he was connected with Francesco Giorgio, an Italian shaman, which directs its customers to Ecuador after preparing for his lectures, courses or "seminars". Emiliano and Denis have befriended a few months earlier, during a meeting of this type. During the flight leading to Caracas where they had to stop before flying to Quito, the two friends were introduced to Clarissa, a young Venezuelan, who showed them some places to visit. On August 5, the day before the disappearance of two young men, the latter received an email written in Spanish where Denis told him Emiliano live with "a strong ritual, with three days of fasting," the time "was" really hard " that he would tell all this verbally to his return, because even in Italian it would be very complicated to explain. Ayahuasca Preparation Fungi ayahausca wnward through the alumina, two zones that fluoresce blue can be spotted by illumination with a black light. The faster-moving zone contains LSD, while the slower-moving zone is iso-LSD. When the zone containing LSD reaches the spigot of the burette, it should be collected in a separate flask. About 3000 ml of the 3-1 benzene-chloroform is required to get the LSD moved down the chromatography column, and finally eluted. The iso-LSD is then flushed from the column by switching the solvent being fed into the top of the column to chloroform. This material is collected in a separate flask, and the solvent removed under a vacuum. The residue is iso-LSD, and should be stored in the freezer until conversion to LSD is undertaken. Directions for this are also given in this chapter. For the fraction containing the LSD, conversion to LSD tartrate must be done to make it water soluble, improve its keeping characteristics, and to allow crystallization. Tartaric acid has the ability to react with two molecules of LSD. Use, then, of a 50% excess of tartaric acid dictates the use of about 1 gram of tartaric acid to 3 grams of LSD. The three grams of LSD would be expected from a well-done batch out of a total 3.5 LSD/iso-LSD mix. The crystalline tartrate is made by dissolving one gram of tartaric acid in a few mis of methanol, and adding this acid solution to the benzene-chloroform elute from the chromatography column. Evaporation of the solvent to a low volume under a vacuum gives crystalline LSD tartrate. Crystals are often difficult to obtain. Instead, an oil may result due to the presence of impurities. This is not cause for alarm; the oil is still likely 90%+ pure. It should be bottled up in dark glass, preferably under a nitrogen atmosphere, and kept in a freezer until moved. If chromatography reveals that one's chosen cooking method produces little of the iso products, then the production of the tartrate salt and crystallization is simplified. The residue obtained at the end Practical LSD Manufacture 32 of the batch is dissolved in a minimum amount of methanol. To this is then added tartaric acid. The same amount is added as above: one gram tartaric acid to three grams LSD. Next, ether is slowly added with vigorous stirring until a precipitate begins to form. The stoppered flask is then put in the freezer overnight to complete the precipitation. After filtering or centrifuging to isolate the product, it is transferred to a dark bottle, preferably under nitrogen, and kept in the freezer until moved. LSD from (so-LSD Two variations on this procedure will be presented here. The first is the method of Smith and Timmis from The Journal of the Chemistry Society Volume 139, H pages 1168-1169 (1936). The other is found in US patent 2,736,728. Both use the action of a strong hydroxide solution to convert iso material into a mixture that contains active and iso material. At equilibrium, the mixture contains about 2/3 Fungi Ayahuasca Kit

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